Mitotically unstable polyploids in the yeast Pichia guilliermondii
✍ Scribed by Dr. U. Klinner; F. Böttcher
- Book ID
- 102388640
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 497 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0233-111X
No coin nor oath required. For personal study only.
✦ Synopsis
Attempts to obtain triploids or tetraploids of P. guillierrnondii by sexual hybridization led to mitotically stable hybrids. However, their DNA content per cell was not higher than in diploids. The results of random spore analysis demonstrate that these hybris were in fact aneuploids which obviously suffered drastic chromosome losses immediately after mating. This phenomenon could have been caused either by aneuploidy already present in the parental strains or it might have been due to a general inability of P. guilliermondii to maintain a polyploid genome.
📜 SIMILAR VOLUMES
Saccharomyces cerevisiae, Canddda rnaltosa and Pichia guilliermondii have been transformcd at different frequencies by the plasmid pYe(ARCT4)411 which carried the Sacch. cereviaiae ARC4 gene inserted into p BR322. Our results strongly suggest that the transformants possessed extrachromosomally maint
BOTTCHXK 'rabio 1 \Vild strains of 1'. yailliervloiidii used in protoplast fusions ~~ ~~ ~n'ild strain Mating type Source 799 llr/lt-