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Transformation of the industrially important yeasts Candida maltosa and Pichia guilliermondii

✍ Scribed by Dr. G. Kunze; C. Petzoldt; R. Bode; I. A. Samsonova; F. Böttcher; D. Birnbaum


Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
260 KB
Volume
25
Category
Article
ISSN
0233-111X

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✦ Synopsis


Saccharomyces cerevisiae, Canddda rnaltosa and Pichia guilliermondii have been transformcd at different frequencies by the plasmid pYe(ARCT4)411 which carried the Sacch. cereviaiae ARC4 gene inserted into p BR322. Our results strongly suggest that the transformants possessed extrachromosomally maintained and autonomously replicating plasmid DNA from which ARG4 gene and p-laetamase gene were expressed.


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