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Mitotically Synchronized Mammalian Cells: A Simple Method for Obtaining Large Populations

✍ Scribed by Robbins, E.; Marcus, P. I.


Book ID
121171999
Publisher
American Association for the Advancement of Science
Year
1964
Tongue
English
Weight
602 KB
Volume
144
Category
Article
ISSN
0036-8075

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A simple procedure to obtain plasmids su
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A simple procedure to obtain plasmid preparations, suitable for transfecting mammalian cell lines using a calcium phosphate co-precipitation technique, is described. The protocol is based on the purification of plasmid DNA by double gel-filtration chromatography on Sephacryl S-1000 and additional sl