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Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence

✍ Scribed by M. Huang; A. K. S. Camara; D. F. Stowe; F. Qi; D. A. Beard

Book ID
106333566
Publisher
Springer
Year
2007
Tongue
English
Weight
472 KB
Volume
35
Category
Article
ISSN
0090-6964

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Rhodamine 123 (Rh123) is widely used as a flow cytometric probe for mitochondrial membrane potential (MMP) in metabolic, pharmacologic, and toxicological studies. However, the use of relatively high concentrations of Rh123 (up to 10 pg/ml) and prolonged incubation times (up to 1 h), including washin