Mitochondrial DNA sequence analysis of the cytochrome oxidase subunit I and II genes, the ATPase9 gene, the NADH dehydrogenase ND4L and ND5 gene complex, and the glutaminyl, methionyl and arginyl tRNA genes fromTrichophyton rubrum

✍ Scribed by Claude Bièvre; Bernard Dujon

• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 708 KB
• Volume: 22
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00351730

✦ Synopsis

In this paper, we present the nucleotide sequence of a 5248 bp-long region of the mitochondrial (rot) genome of the dermatophyte Trichophyton rubrum.

This region which represents about 1/4 of the total mt genome of this species reveals a compact organization of genes including: the glutaminyl tRNA, the methionyl tRNA, the cytochrome oxidase subunit I gene, the arginyl tRNA, the mitochondrial version of the ATPase subunit 9 gene, the cytochrome oxidase subunit II gene and a part of the NADH dehydrogenase ND4L and ND5 gene "complex". The main features of the part of mt DNA sequenced is the non-interrupted COX I gene and the presence in the mitochondrial version of the ATPase 9 gene of a small group IA intron. The extensive aminoacid sequence similarity with the equivalent gene in Aspergillus nidulans and Neuropora crassa indicates that this gene codes for a dicyclohexylcarbodiimide binding protein. The conserved arrangement of this portion of the mt genome and the presence of tRNAs between the protein-coding genes are compatible with a large polycistronic transcript processed by the excision of tRNAs, or similar secondary structures, as proposed for other fungal or mammalian mt DNAS.