Analysis of a DNA segment from rat liver mitochondria containing the genes for the cytochrome oxidase subunits I, II and III, ATPase subunit 6, and several tRNA genes

✍ Scribed by Rüdiger Grosskopf; Horst Feldmann

• Publisher: Springer-Verlag
• Year: 1981
• Tongue: English
• Weight: 686 KB
• Volume: 4
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00365694

✦ Synopsis

Goethestrasse 33, 8000 M/inchen 2, Federal Republic of Germany Summary. The sequence of a 6.24 kb DNA segment of the mitochondrial genome from rat liver has been determined. It comprises several genes coding for mitochondrial protein subunits and five tRNA genes in the followset ing order: cytochrome oxidase subunit I-tRNA(ucNI -tRNA Asp -cytochrome oxidase subunit II -tRNA Lys -ATPase subunit -cytochrome oxidase subunit III -tRNA Gly -potential open reading frame -tRNA Argtwo potential open reading frames. The tRNA genes were detected by a computer search programme. The assignments for the protein coding sequences were made through comparison with known sequences, mainly from the yeast mitochondrial proteins (e.g. Bonitz et al. 1980). Our data are discussed with regard to the features of gene arrangement, codon usage, and tRNA structure in mammalian mitochondria (Anderson et al. 1981).