Mitochondria-involved apoptosis induced by MPPa mediated photodynamic therapy

✍ Scribed by Y.Y. Tian; D.D. Xu; X. Tian; F.A. Cui; H.Q. Yuan; W.N. Leung

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 96 KB
• Volume: 5
• Category: Article
• ISSN: 1612-2011
• DOI: 10.1002/lapl.200810052

✦ Synopsis

Numerous new photosensitizers are now in various stages of trials demonstrating the broad applicability of Photodynamic therapy (PDT). However, only a handful of photosensitizers have received regulatory approval. Lack of effective photosensitizers has become a major limit for extensive application of PDT. Our previous study showed MPPa to be a good photosensitizer candidature, MPPa-PDT can lead PC-3M cell line to death mainly via apoptotic way both in vitro and in vivo, and part of the mechanism was investigated. Mitochondria may play a key role in the process, in order to further elucidate the mechanism, we investigated the level of ROS, GSH, NO, Ca 2+ , mitochondrial membrane potential, as well as cytochrome C. All in all, ROS production, depletion of GSH, and the activation of ROS downstream, such as mitochondria depolarization, cytochrome C release, were detected in our study. The results provide a mechanism by which oxidative stress provokes apoptosis of PC-3M cells. GSH, mmol/mg protein 140 120 100 80 60 40 20 0 0 10 20 40 60 80 0.5 μM 1 μM 2 μM 4 μM GSH in PC-3M 30 minutes after PDT. Cells were incubated for 12 h with various concentrations of MPPa and then irradiated with different light dose. Protein concentration was measured by the method of Bradford. Results are expressed as mean ± SD