<p><p>This volume provides a concise and technical discussion of recently developed approaches to overcome challenges, such as pharmacodynamics and pharmacokinetics difficulties, in miRNA drug discovery. These strategies cover anti-sense agents targeting miRNA that are applied in advanced formulatio
miRNA Maturation: Methods and Protocols
β Scribed by Michael Sand (auth.), Christoph Arenz (eds.)
- Publisher
- Humana Press
- Year
- 2014
- Tongue
- English
- Leaves
- 197
- Series
- Methods in Molecular Biology 1095
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.
β¦ Table of Contents
Front Matter....Pages i-x
Front Matter....Pages 1-1
Front Matter....Pages 3-10
Back Matter....Pages 11-25
....Pages 27-58
β¦ Subjects
Human Genetics; Gene Expression
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