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miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs

✍ Scribed by Xie, Fuliang; Xiao, Peng; Chen, Dongliang; Xu, Lei; Zhang, Baohong


Book ID
118808299
Publisher
Springer
Year
2012
Tongue
English
Weight
341 KB
Volume
80
Category
Article
ISSN
0167-4412

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A small-scale method for the isolation of total RNA from plant tissue is described. The method provides RNA of suitable quantity and quality from 0.2 g fresh tissue for the detection of mRNA species by RNA blot analysis. The entire procedure is adapted to 1.5-ml microfuge tubes and takes less than 5