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Minireview: Isoelectric focusing

โœ Scribed by Michel Prats


Book ID
103703167
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
434 KB
Volume
20
Category
Article
ISSN
0307-4412

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Analytical isoelectri& focusing of peptides in 0.7~mm thick gel slabs, in presence of 8 M urea, is made possible by staining the gel directly in Coomassie Brilliant Blue G-250 dissolved in 1 N H2S0,12% trichloroacetic acid solution. The carrier ampholytes are soluble in this solvent whereas the pept