𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Minimal deletion of 3p13→14.2 associated with immortalization of human uroepithelial cells

✍ Scribed by Lydia Vieten; Cassandra D. Belair; Lena Savelieva; Knut Jülicher; Frank Bröcker; Walter Bardenheuer; Jochen Schütte; Bertram Opalka; Catherine A. Reznikoff

Book ID
101260895
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
262 KB
Volume
21
Category
Article
ISSN
1045-2257

No coin nor oath required. For personal study only.

✦ Synopsis

Immortalization and tumorigenic transformation of many human cell types, including human uroepithelial cells (HUCs), are frequently associated with loss of genetic material from the short arm of chromosome 3 (3p). In addition, losses of 3p have been observed in many human cancers including renal cell carcinoma, lung cancer, breast cancer, and bladder cancer. Genetic studies suggest that there are at least two regions on 3p in which tumor suppressor genes might be located, but the precise location of these genes is not known. We studied chromosome 3 losses that were specifically associated with immortalization of five independent human papilloma virus 16 (HPV16) E6-or E7-transformed HUCs. Cytogenetic analysis showed that the smallest common region of deletion was 3p14.1=14.2. Fluorescence in situ hybridization using a 3p13=14-specific yeast artificial chromosome (YAC) contig showed the precise localization of the breakpoints to be in 3p13 and 3p14.2, thus defining the smallest common overlap of 3p deletions in HPV16 E6-or E7-immortalized HUCs. These results suggest the presence in this region of genes involved in the control of senescence in vitro and possibly tumorigenesis in vivo.

📜 SIMILAR VOLUMES

Chromosome bands 3p14.2, 9p21, and 13q14
Chromosome bands 3p14.2, 9p21, and 13q14 are frequently deleted in roentgenographically occult bronchogenic squamous cell carcinoma of the lung
✍ Masami Sato; Akira Sakurada; Motoyasu Sagawa; Chiaki Endo; Tatsuo Tanita; Takash 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 89 KB 👁 1 views

Roentgenographically occult bronchogenic squamous cell carcinomas are early lung cancers that localize mainly within the bronchial wall and are thought to be a good model for elucidating chromosomal alterations during lung cancer progression. In this study, we analyzed allelic losses on chromosome r

Immortalization of human WI38 cells is a
Immortalization of human WI38 cells is associated with differential activation of the c-myc origins
✍ Liang Tao; Zhifeng Dong; Maria Zannis-Hadjopoulos; Gerald B. Price 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 251 KB

## Abstract To study the possible relationships between origin activities and cellular processes leading to malignancy, we used an isogenic system of human embryo lung fibroblast cells WI38 and a SV40‐transformed variant, WI38 VA13 2RA (WI38(SV40)). We found that the activities of all initiation si

Biallelic deletion within 16p13.13 inclu
Biallelic deletion within 16p13.13 including SOCS-1 in Karpas1106P mediastinal B-cell lymphoma line is associated with delayed degradation of JAK2 protein
✍ Ingo Melzner; Marc A. Weniger; Alexandra J. Bucur; Silke Brüderlein; Karola Dors 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 211 KB

## Abstract Activity of Janus kinase 2 (JAK2) in the JAK2/STAT5 signaling pathway is critically controlled by suppressor of cytokine signaling‐1 (SOCS‐1). We have previously shown that __SOCS‐1__ is biallelically mutated in the primary mediastinal B‐cell lymphoma (PMBL) cell line MedB‐1, resulting