Microsystem for the immunomagnetic detection ofEscherichia coliO157:H7

✍ Scribed by Mujika, M. ;Arana, S. ;Castaño, E. ;Tijero, M. ;Vilares, R. ;Ruano-López, J. M. ;Cruz, A. ;Sainz, L. ;Berganza, J.

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 923 KB
• Volume: 205
• Category: Article
• ISSN: 0031-8965
• DOI: 10.1002/pssa.200778126

✦ Synopsis

Abstract

A hand held microsystem has been designed for the immunomagnetic detection and quantification of the pathogen Escherichia coli O157:H7 in food and clinical samples (see abstract figure). In this work a technology that facilitates the integration of 3D microfluidic networks with any microfabricated biosensor to manufacture a Lab On a Chip has been developed. With this aim, the design of the microfluidic circuitry, the sensing film optimization, the packaging needed to carry out the assays in a safe and straightforward way and, finally, the development of the biological protocols involved in the measurements have been completed. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)