Microsurgical repair after crush-avulsion injury of the femoral vein in rats: Prevention of microvascular thrombosis with recombinant human tissue-type plasminogen activator (rt-PA)

✍ Scribed by Efstathios G. Lykoudis; George B. Contodimos; Dimosthenis A. Tsoutsos; Konstantina B. Frangia; Apostolos E. Papalois; Constantinos N. Stamatopoulos; John D. Ioannovich

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 284 KB
• Volume: 21
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/micr.21808

✦ Synopsis

Abstract

Vein thrombosis is often encountered in microsurgery, especially in the case of crush‐avulsion injuries. The aim of this study was to investigate the effect of systemic administration of recombinant tissue‐type plasminogen activator (rt‐PA) on the patency of the femoral vein of the rat, which had previously sustained a crush‐avulsion injury. The study consisted of 3 groups of male Wistar rats, 20 animals each. A standardized crush‐avulsion injury model was used. After microvascular repair of the femoral vein, the animals received either normal saline (group A), heparin 100 U/kg body weight (group B), or rt‐PA 3.5 mg/kg body weight (group C) systemically. Patency tests were performed at 20 minutes, 48 hours, and 1 week after blood flow reestablishment. According to our results, the patency rate of the rt‐PA group was significantly higher than in both the control and heparin groups. © 2001 Wiley Liss, Inc. MICROSURGERY 21:357–361, 2001