Microsurgery in a rat lung transplant model: Analysis of benefit

✍ Scribed by Osvaldo J. Yano; Robin Baradarian; Eugene S. Kim; Tami J. Smith; Mark L. Smith; Craig R. Smith; David T. Chiu

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 457 KB
• Volume: 17
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/(sici)1098-2752(1996)17:1<25::aid-micr5>3.0.co;2-v

✦ Synopsis

With the use of microsurgery, we have developed a method of measuring hemodynamic parameters in a rat not possible with previous technology. Three groups of rats were studied: a chemically induced pulmonary hypertensive group (PH); a chemically induced pulmonary hypertensive group treated with single lung transplantation (LT); and an untreated, control group (C). Cardiac output, heart rate, and pulmonary vascular resistance were then calculated in each group from data obtained by 1 mm high fidelity micromanometers and an ultra-sonic flow probe. The results show that the data col-lected from the rodent model are reproducible within each group, and data quality is comparable to large animal models. With this new method, data can be collected in a small animal model at a fraction of the time and cost of large animal studies. Additionally, the complications of graft rejection in large animal studies are eliminated in an isogenic rodent model.