Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis
✍ Scribed by Christov, Alexander; Dai, Erbin; Liu, Liying; Miller, Leslie W.; Nash, Piers; Lalani, Alshad; McFadden, Grant; Nation, Patric N.; Tulip, John; Lucas, Alexandra
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 739 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Background and Objective
: Transplant vasculopathy is a leading cause of late cardiac graft loss. We have examined laserinduced fluorescence (LIF) spectroscopy as an optical diagnostic tool for detection of intimal plaque development and inflammatory cellular invasion in a rat model of aortic allograft transplant. Study Design/Materials and Methods: Infrarenal aortic segments were transplanted from Lewis to Sprague Dawley rats. A range of vasculopathy development was produced by treatment with a viral anti-inflammatory protein. LIF spectra were recorded from the intima of aortic implants at 28 days. Fluorescence intensity was analyzed for correlation with vasculopathy development. Results: Significant differences in LIF intensity at 400-450 nm (P ≤ 0.05 by ANOVA) were detected. LIF emission was correlated with plaque growth (R 2 = 0.980), vessel narrowing (R 2 = 0.964), and cellular invasion (R 2 = 0.971) by regression analysis. Conclusion: LIF optical analysis provides a nontraumatic diagnostic approach for detection of atherosclerosis prior to cardiac transplant or during development of vasculopathy after transplant.