Hypocotyl segments of 2- to 3-week-old radish (Raphanus sativus L. cv. F1 Handsome Fall) seedlings produced yellowish compact calli when cultured on Murashige and Skoog's (MS) medium supplemented with 1 mgl(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Upon transfer onto medium containing 6-benzyladen
Microspore culture of radish (Raphanus sativusL.): influence of genotype and culture conditions on embryogenesis
โ Scribed by Yoshihito Takahata, Hisashi Komatsu, Norihiko Kaizuma
- Book ID
- 120779771
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 395 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
In microspore cultures of Brassica napus L. cv. Topas, embryo yield increases with culture density up to about 40,000 microspores per ml. A much higher density (100,000 per ml) appears inhibitory to embryogenesis. A relatively high culture density (30,000 or 40,000 per ml) for the first 2-4 days of
Production of microspore-derived embryos from cultured anthers is now a well established technique for the isolation of homozygous lines in many crop plants. We describe here a culture method for embryo induction and plant regeneration from anthers of four sunflower genotypes. For preliminary experi