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Effect of incubation temperature regimes and culture medium on broccoli microspore culture embryogenesis

✍ Scribed by João Carlos da Silva Dias

Book ID
110363554
Publisher
Springer
Year
2001
Tongue
English
Weight
39 KB
Volume
119
Category
Article
ISSN
0014-2336

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✍ Bin Huang; Sharon Bird; Roger Kemble; Daina Simmonds; Wilf Keller; Brian Miki 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 509 KB

In microspore cultures of Brassica napus L. cv. Topas, embryo yield increases with culture density up to about 40,000 microspores per ml. A much higher density (100,000 per ml) appears inhibitory to embryogenesis. A relatively high culture density (30,000 or 40,000 per ml) for the first 2-4 days of