Microsequencing of peptides and proteins
β Scribed by A.D. Strosberg; L. Camoin; J.-L. Guillaume
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 851 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0165-9936
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A novel apparatus for performing manual gas-phase Edman chemistry on protein and peptide samples is described. Edman chemistry is performed in 6 to 10 Teflon continuous flow reactors (CFR), previously described by J. E. Shively et al. (1987) Anal. Biochem. 163, 5 17-529). The CFRs are packed with IO
Attachment studies with EDC (1 -ethyl-3-dimethylaminopropyl carbodiimide) have revealed that peptides, ranging from 4 to 31 residues, can be linked to aminopropyl glass with satisfactory yield, if optimal conditions are obeyed. The attachment yields are, however, significantly lower with peptides po