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Accelerated high-sensitivity microsequencing of proteins and peptides using a miniature reaction cartridge

✍ Scribed by Nicholas F. Totty; J. Justin Hsuan; Michael D. Waterfield

Publisher
Cold Spring Harbor Laboratory Press
Year
1992
Tongue
English
Weight
814 KB
Volume
1
Category
Article
ISSN
0961-8368

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✦ Synopsis

Abstract

Greatly reduced automated protein sequencing degradation times of less than 25 min with concurrent on‐line phenylthiohydantoin (PTH) derivative analysis times of less than 16 min have been achieved by using a miniaturized reaction cartridge with optimized chemical and analytical cycles. Using this method a wide range of standard and novel peptides and proteins have been sequenced with reproducibly high initial and repetitive cycle yields. In these accelerated analyses the recovery of the more labile PTH derivatives was markedly improved by using elevated pressure during cleavage steps and temperature programming throughout the Edman cycle.

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