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High-performance capillary electrophoretic separation of proteins and peptides using a bonded hydrophilic phase capillary

✍ Scribed by Giovanni Piccoli; Mara Fiorani; Beatrice Biagiarelli; Francesco Palma; Luciana Vallorani; Roberta De Bellis; Prof. Vilberto Stocchi

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 384 KB
Volume: 16
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.11501601100

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✦ Synopsis

High-performance capillary electrophoretic separation of proteins and peptides using a bonded hydrophilic phase capillary

High performance capillary electrophoresis (HPCE) was applied to the separation of protein and peptide mixtures with molecular masses ranging from 1300 to 96000 Da using a new bonded hydrophilic phase capillary, CElect-P15O. This coated capillary reduces the interaction between proteins and silanol groups in capillary walls, allowing a complete recovery of the proteins and peptides of interest. HPCE was also used for the analysis of a complex mixture of tryptic fragments and to monitor the process of enzymatic digestion. Moreover, using a CElect-P15O capillary, highly reproducible analysis was possible without preconditioning the capillary with acid or basic solutions before each new analysis.

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