Capillary electrophoretic separation of the enantiomers of weak acids in a high pH background electrolyte using the new, single-isomer, octakis(2,3-diacetyl-6-sulfato)-γ-cyclodextrin as chiral resolving agent

✍ Scribed by Wenhong Zhu; Gyula Vigh

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 429 KB
• Volume: 12
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(2000)12:3<167::aid-mcs6>3.0.co;2-l

✦ Synopsis

The new, single-isomer, octakis 2,3-diacetyl-6-sulfato -␥-cyclodextrin Ž . ODAS-␥ CD has been used for the capillary electrophoretic separation of the enantiomers of weak acids, mostly nonsteroidal anti-inflammatory agents, in a high pH background electrolyte where all the acidic analytes were fully dissociated. The anionic effective mobilities of the weakly-binding anionic analytes decreased with increasing concentration of ODAS-␥ CD, while those of the strongly binding analytes passed a local minimum due to the combined, opposing effects of complexation and ionic strength-induced mobility depression. Separation selectivity decreased as the concentration of ODAS-␥ CD was increased, in agreement with the predictions of the charged resolving agent migration model. Good peak resolution could be obtained with ODAS-␥ CD in less than 10-20 min for all the weak acid analytes tested. ᮊ 2000