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Microsequence analysis of peptides and proteins: IV. Structural studies on human leukocyte interferons

✍ Scribed by John E. Shively; Ursino Del Valle; Russell Blacher; David Hawke; Warren P. Levy; Menachem Rubinstein; Stanley Stein; Courtney McGregor; Joseph Tarnowski; Robert Bartlett; David Lee; Sidney Pestka

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
578 KB
Volume
126
Category
Article
ISSN
0003-2697

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✦ Synopsis

The sequence of the ttyptic peptides of three major species of human leukocyte interferon was determined by microsequencing procedures. The peptides were aligned by comparison with the amino acid sequences predicted by the DNA sequences of recombinants containing leukocyte interferon-coding inserts. In addition, extended NH2-terminal amino acid sequences of two human leukocyte intetferons produced in Escherichia coli by recombinant DNA methodology are also reported. This report demonstrates application of microsequencing methodology to low nanomole and subnanomole amounts of proteins and peptides of biological interest.

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