## Microscale Separation Methods for Metabolomics The metabolome is the complete set of endogenous low-molecular-weight metabolites in cells, tissues, body fluids and plants. The comprehensive analysis of these metabolites in biological samples is known as metabolomics. Metabolomics is particularl
Microscale separation methods for enzyme kinetics assays
✍ Scribed by Křížek, Tomáš; Kubíčková, Anna
- Book ID
- 113021867
- Publisher
- Springer
- Year
- 2012
- Tongue
- English
- Weight
- 353 KB
- Volume
- 403
- Category
- Article
- ISSN
- 1618-2650
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A new kinetic method is described for the assay of quinone-reducing enzymes in various biological materials. It is based on polarographic determination of oxygen uptake in spontaneous oxidation of the diphenol formed as a result of 4-anilino-5-methoxybenzoquinone-1 ,2 (AMOBQ) enzymic reduction. The
A stainless-steel cylindrical vessel is described with optical ports which can be pressurized to 1000 atm (hydrostatic). Constituents for enzymic reactions are injected directly into the vessel, sealed without use of tools, and pressurized. All these operations can be completed in about 10 sec. The