Micropreparative Gel Electrophoresis of Low-Molecular-Weight Peptides: Purification of Highly Insoluble Amyloid Peptide Fragments

Application of the dansylation reaction

Application of the dansylation reaction to the characterization of low molecular weight peptides by dodecyl sulfate-polyacrylamide-gel electrophoresis

✍ Taiji Kato; Makoto Sasaki; Shigeru Kimura 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 508 KB

Polyacrylamide-gel electrophoresis of low molecular weight DNS-peptides was performed in the presence of 0.1% sodium dodecyl sulfate and 8 M urea. This procedure enabled an estimation of the molecular weight of peptides at the nanomole level without staining. A linear relationship between molecular