Micromethod for the quantitative determination of l-alanine

✍ Scribed by Akira Yoshida

• Publisher: Elsevier Science
• Year: 1965
• Tongue: English
• Weight: 208 KB
• Volume: 11
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(65)90028-x

✦ Synopsis

Micromethod

for the Quantitative Determination of L-Alanine Alanine exists in nature as both L and D isomers, particularly in bacterial cell walls. It is occasionally necessary to quantitatively measure L-alanine in a sample containing n-alanine as well as ot,her compounds. However, no satisfactory direct and accurate method for the determination of L-alanine has been reported.

Recently n-alanine dehydrogenase (L-alanine : KAD oxidoreductase, deaminating, EC 1.4.1.1, AlD) was purified in crystalline form from Racillus subtilis

(1). By utilizing its high activity and specificity for L-alanine, it is now possible to determine micro amounts of L-alanine even in the presence of n-alanine and other compounds.