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Microglia: Methods and Protocols

✍ Scribed by Olga Garaschuk, Alexei Verkhratsky

Publisher
Springer New York;Humana
Year
2019
Tongue
English
Leaves
332
Series
Methods in Molecular Biology 2034
Edition
1st ed. 2019
Category
Library
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✦ Synopsis

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and “omics” analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.

✦ Table of Contents

Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
Microglia: The Neural Cells of Nonneural Origin (Olga Garaschuk, Alexei Verkhratsky)....Pages 3-11
Morphology of Microglia Across Contexts of Health and Disease (Julie C. Savage, Micaël Carrier, Marie-Ève Tremblay)....Pages 13-26
Physiology of Microglia (Olga Garaschuk, Alexei Verkhratsky)....Pages 27-40
Microglia Reactivity: Heterogeneous Pathological Phenotypes (Hélène Hirbec, François Rassendren, Etienne Audinat)....Pages 41-55
Microglia in Neurodegenerative Disorders (Darío Tejera, Michael T. Heneka)....Pages 57-67
Physiological Implications of Microglia–Synapse Interactions (Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, Junichi Nabekura)....Pages 69-80
Front Matter ....Pages 81-81
Ultrastructural Analyses of Microglial Interactions with Synapses (Marie-Ève Tremblay, Ania K. Majewska)....Pages 83-95
Visualizing Dark Microglia (Marie-Kim St-Pierre, Maude Bordeleau, Marie-Ève Tremblay)....Pages 97-110
Electrophysiological Investigation of Microglia (Elena Avignone, Giampaolo Milior, Isabelle Arnoux, Etienne Audinat)....Pages 111-125
Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia (Annette Masuch, Knut Biber)....Pages 127-147
Assessment of Parasite–Microglia Interactions In Vitro (Katherine Figarella, Nestor L. Uzcategui, Michael Duszenko)....Pages 149-161
Front Matter ....Pages 163-163
In Vivo Visualization of Microglia Using Tomato Lectin (Bianca Brawek, Maria Olmedillas del Moral, Olga Garaschuk)....Pages 165-175
Long-Term In Vivo Imaging of Individual Microglial Cells (Angelos A. Skodras, Jasmin K. Hefendehl, Jonas J. Neher)....Pages 177-189
Analysis of Signaling Mechanisms Regulating Microglial Process Movement (Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, Christian Madry)....Pages 191-205
Measuring Microglial Turnover in the Adult Brain (Diego Gomez-Nicola, Gemma L. Fryatt, Katharine E. Askew)....Pages 207-215
Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor (Maja Kitic, Peter See, Julia Bruttger, Florent Ginhoux, Ari Waisman)....Pages 217-230
Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia (Bianca Brawek, Olga Garaschuk)....Pages 231-241
Labeling Microglia with Genetically Encoded Calcium Indicators (Yajie Liang, Olga Garaschuk)....Pages 243-265
Calcium Imaging of Microglial Network Activity in Stroke (Petr Tvrdik, Kathryn N. Kearns, Khadijeh A. Sharifi, M. Filip Sluzewski, Scott T. Acton, M. Yashar S. Kalani)....Pages 267-279
In Vivo Two-Photon Imaging of Microglial Synapse Contacts (Daisuke Kato, Ako Ikegami, Hiroshi Horiuchi, Andrew J. Moorhouse, Junichi Nabekura, Hiroaki Wake)....Pages 281-286
In Vivo Phagocytosis Analysis of Amyloid Beta (Darío Tejera, Michael T. Heneka)....Pages 287-292
Functional Analysis of Brain-Engrafted Monocytes After Microglia Ablation in Mouse Models (Nicholas H. Varvel, Richard M. Ransohoff, Jonas J. Neher)....Pages 293-301
Front Matter ....Pages 303-303
Analysis of the Microglial Sensome (Suzanne E. Hickman, Joseph El Khoury)....Pages 305-323
Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue (Giampaolo Milior, Farah Chali, Tiffany Dos Santos, Juliette Royer, Richard Miles, Mélanie Morin-Brureau)....Pages 325-336
Back Matter ....Pages 337-340

✦ Subjects

Biomedicine; Neurosciences; Immunology

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