Aqueous Two-Phase Systems: Methods and Protocols: Methods and Protocols

✍ Scribed by Rajni Hatti-Kaul (auth.), Rajni Hatti-Kaul (eds.)

• Publisher: Humana Press
• Year: 2000
• Tongue: English
• Leaves: 441
• Series: Methods in Biotechnology™ 11
• Edition: 1
• Category: Library

✦ Synopsis

In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented.
Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

✦ Table of Contents

Front Matter....Pages i-xiii
Front Matter....Pages 1-10
Front Matter....Pages 11-21
Front Matter....Pages 23-33
Front Matter....Pages 35-45
Front Matter....Pages 11-45
Back Matter....Pages 47-54
....Pages 55-64

✦ Subjects

Biotechnology