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Microenvironmental effect of stabilizing polylectroytes in ultrafiltration membrane enzymatic reactors

✍ Scribed by Liliana Gianfreda; Domenico Pirozzi; Guido Greco Jr.

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
431 KB
Volume
33
Category
Article
ISSN
0006-3592

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✦ Synopsis

Complex bioreaction patterns are usually dealt with in immobilized cell reactors, rather than in immobilized multi-enzyme systems. This is somewhat surprising, since the latter could provide much better results, at least in principle. Indeed, the absence of enzymes not involved directly in the conversion would insure higher selectivities and, furthermore, artificial metabolic pathways could be assembled, by making use of enzymes from different sources.

Many difficulties arise, however, in dealing with immobilized multi-enzyme systems, related to:

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Improved operational stability of cell-free glucose-fructose oxidoreductase from Zymomonas mobilis for the efficient synthesis of sorbitol and gluconic acid in a continuous ultrafiltration membrane reactor
✍ Bernd Nidetzky; Monika FΓΌrlinger; Dorothee Gollhofer; Robert K. Scopes; Dietmar πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 177 KB πŸ‘ 2 views

For the continuous, enzymatic synthesis of sorbitol and gluconic acid by cell-free glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion