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Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. III. Utilization of two thermostable β-glycosidases in a continuous ultrafiltration membrane reactor and galacto-oligosaccharide formation under steady-state conditions

✍ Scribed by Inge Petzelbauer; Barbara Splechtna; Bernd Nidetzky


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
194 KB
Volume
77
Category
Article
ISSN
0006-3592

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Development of an ultra-high-temperature
✍ Inge Petzelbauer; Reinhard Zeleny; Andreas Reiter; Klaus D. Kulbe; Bernd Nidetzk 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 188 KB 👁 1 views

During lactose conversion at 70°C, when catalyzed by ␤-glycosidases from the archea Sulfolobus solfataricus (Ss␤Gly) and Pyrococcus furiosus (CelB), galactosyl transfer to acceptors other than water competes efficiently with complete hydrolysis of substrate. This process leads to transient formation