✦ LIBER ✦

Microencapsulation selection for isolation of yeast mutants with increased secretion of Aspergillus awamori glucoamylase

✍ Scribed by K. D. Wittrup; A. L. Weber; P. Tsai

Book ID: 102768777
Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 603 KB
Volume: 42
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260420312

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✦ Synopsis

We have developed a microencapsulation selection method which allows the rapid and quantitative screening of >lo6 yeast cells for enhanced secretion of Aspergillus awamori glucoamylase. The method provides a 400-fold single-pass enrichment for highsecreting mutants, and can be straightforwardly adapted for application to growth-based selection schemes with other microorganisms and enzymes.

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