## Abstract The use of peroxidase‐conjugated antibodies for detecting specific tumor associated antigens bound to nitrocellulose membranes is being increasingly used in our laboratory. This method offers many advantages. However, we have observed several problems which can cause confusing results a
Microelectrophoresis of serum proteins on nitrocellulose membranes
✍ Scribed by T.I. Přistoupil; V. Fričová
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 312 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Radial chromatography of serum proteins was investigated under various experimental conditions on nitrocellulose membranes. Development of about 15 ,ug/o.s ~1 samples on Sartorius 1x002 filters at pH 8.0-9.0, and with a salt concentration of up to 5 o/o NaCl at laboratory temperature (18-25") w
Proteins are biotinylated after immobilization on nitrocellulose sheets by reaction with a biotinyl-succinimide ester. The biotinyl residues are visualized by streptavidin-peroxidase-based detection systems either by deposition of a colored formazan dye or by enhanced chemiluminescence (ECL), the la