𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Microassay of protein with nitrocellulose membrane filters

✍ Scribed by Hirozi K. Kihara; Harumi Kuno

Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
544 KB
Volume
24
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Microassay for proteins on nitrocellulos
Microassay for proteins on nitrocellulose filter using protein dye-staining procedure
✍ Kazuyuki Nakamura; Tatehiko Tanaka; Akira Kuwahara; Kazusuke Takeo πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 732 KB

A simple and rapid microassay for proteins utilizing the protein dye-staining procedure with a nitrocellulose (NC) filter is described. Proteins were directly bound to an NC filter using the "BIO DOT" microfiltration apparatus to ensure their uniformity. The proteins were then stained with a dye (Po

A simple assay of solubilized adenosine
A simple assay of solubilized adenosine receptors with nitrocellulose membrane filters
✍ Hiroyasu Nakata; Hitoshi Fujisawa πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 384 KB

A rapid and simple assay of solubilized adenosine receptors with nitrocellulose membrane filters is described. This assay was sensitive and reproducible when applied to adenosine receptors solubilized from rat brainstem membranes with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Approp

Routine detection of calcium-binding pro
Routine detection of calcium-binding proteins following their adsorption to nitrocellulose membrane filters
✍ Maxwell T. Hincke πŸ“‚ Article πŸ“… 1988 πŸ› Elsevier Science 🌐 English βš– 671 KB

A routine semiquantitative procedure which permits soluble calcium-binding proteins to be detected following their adsorption to nitrocellulose membrane filters by liquid scintillation counting of specifically bound 45Ca is described. Proteins with high affinity for calcium such as calmodulin and tr

A solid-phase assay for the phosphorylat
A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters
✍ Flavia Valtorta; Werner Schiebler; Reinhard Jahn; Bruno Ceccarelli; Paul Greenga πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 979 KB

A new procedure for the phosphorylation and assay of phosphoproteins is described. Proteins are solubilized from tissue samples, separated by polyacrylamide gel electrophoresis, transferred onto nitrocellulose membrane filters, and the blotted polypeptides are phosphorylated with the catalytic subun

Microassay of proteins on membrane filte
Microassay of proteins on membrane filter in the nanogram range using cycloheptaamylose-dansyl chloride complex
✍ Toshio Kinoshita; Fumio Iinuma; Akio Tsuji πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 365 KB

A protein assay is described in which the sample is spotted on a Sartorius membrane filter and dansylated with cycloheptaamylose-dansyl chloride complex dissolved in aqueous urea solution. The fluorescence intensity of the spot on the membrane was directly measured by a scanning fluorometer. This as