Microelectrochemical radioiodination of monoclonal antibody: A preliminary study

✍ Scribed by Z. M. Wong; F. W. Teare; B. M. Bowen; S. K. Liao; C. S. Kwok; P. C. Kwong; I. Boxen

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: French
• Weight: 325 KB
• Volume: 24
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580241205

✦ Synopsis

A P r e l i m i n a r y S t u d y Z.M. Wong(l), F . W . T e a r e ( l ) , B.M. Bowen(l,2), S.K. Liao(3). C.S. Kwok(41, P.C. Kvong(3), and I. B o x e n ( 5 ) ( 1 ) U n i v e r s i t y of T o r o n t o , F a c u l t y o f P h a r m a c y , 19 R u s s e l l S t r e e t , T o r o n t o , O n t a r i o , C a n a d a , 1 5 s 1Al ( 2 ) C h e d o k e -M c M a s t e r Hospitals. D e p a r t m e n t of N u c l e a r M e d i c i n e , H a m i l t o n , O n t a r i o ( 3 ) M c M a s t e r U n i v e r s i t y M e d i c a l C e n t e r , D e p a r t m e n t s of P e d i a t r i c s L P a t h o l o g y , H a m i l t o n , O n t a r i o (4)Ontario C a n c e r R e s e

a r c h F o u n d a t i o n , H a m i l t o n C l i n i c , H a m i l t o n , O n t a r i o ( 5 ) H e n d e r s o n G e n e r a l H o s p i t a l , D e p a r t m e n t o f N u c l e a r M e d i c i n e , H a m i l t o n , O n t a r i o S U M M A R Y T h e o p t i m a l r e a c t i o n c o n d i t i o n s for t h e m i c r o e l e c t r o c h e m i c a l i o d i n a t i o n o f i m m u n o g l o b u l i n s w e r e d e t e r m i n e d w i t h non-spocific h u m a n serum immunoglobulins. T h e s e c o n d i t i o n s w e r e u s e d for t h e e f f i c i e n t r a d i o i o d i n a t i o n of a m o n o c l o n a l a n t i b o d y , 140.240, i n s u b m i l l i g r a m quantities. A n a p p r o x i m a t e l y five-fold d e c r e a s e i n t h e t i t r e o f t h e a n t i b o d y a g a i n s t m e l a n o m a c e l l s , a s d e t e r m i n e d by the m i n i a t u r i z e d m i x e d h e m a d s o r p t i o n a s s a y , w a s o b s e r v e d a f t e r i o d i n a t i o n w i

t h a n a v e r a g e o f 0.85 a t o m s of iodine per m o l e c u l e o f antibody. KEY WORDS: Iodination E f f i c i e n c y , I m m u n o g l o b u l i n s , E l e c t r o c h e m i c a l , M o n o c l o n a l A n t i b o d y , Iodine-125 I N T R O D U C T I O N E l e c t r o c h e m i c a l i o d i n a t i o n h a s b e e n used t o p r e p a r e iodinated p r o t e i n s of h i g h s p e c i f i c a c t i v i t y and s a t i s f a c t o r y q u a l i t y f o r r a d i o i m m u n o a s s a y , r a d i o i m m u n o d e t e c t i o n and t r a c e r studies. T h e u s e o f p l a t i n u m e l e c t r o d e s m i n i m i z e s e l e c t r o d e r e a c t i o n s ( 1 ) so t h a t t h e r e i s m i n i m a l c h e m i c a l d a m a g e t o proteins, e v e n t h o u g h they c o m e i n t o c o n t a c t w i t h t h e e l e c t r o d e 0362-4soUslll21435-lOSOS.00 @ 1987 by John Wiley & Sons, Ltd.