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Microdetermination of glycerol using bacterial NADH-linked luciferase

✍ Scribed by Horst Kather; Friedhelm Schröder; Bernd Simon


Book ID
115824091
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
422 KB
Volume
120
Category
Article
ISSN
0009-8981

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A method is described determining the pyruvate content in l-10 pg of lyophilized tissue. This represents a range of weight much below that of fine-needle biopsies. NAD+, generated through the enzymatic conversion of pyruvate to lactate, is reduced to NADH which is then assayed by its ability to indu