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Properties of bacterial luciferase/NADH : FMN oxidoreductase and firefly luciferase immobilized onto sepharose

✍ Scribed by Gabriele K. Wienhausen; Larry J. Kricka; John E. Hinkley; Marlene DeLuca

Book ID
112896537
Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
774 KB
Volume
7
Category
Article
ISSN
0273-2289

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## Abstract A modified purification method for bacterial luciferases and NAD(P)H:FMN oxidoreductases is described which uses FMN‐Sepharose alone or coupled to DEAE ion exchange chromatography for the simultaneous purification of luciferase and the various oxidoreductases from __Vibrio harveyi__, a