๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Microcytotoxicity blocking assay for the detection and isolation of soluble astrocytoma association antigen

โœ Scribed by Virginia P. Potter; William C. Wood; Alfred M. Cohen; Paul L. Kornblith

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
454 KB
Volume
26
Category
Article
ISSN
0022-4790

No coin nor oath required. For personal study only.

โœฆ Synopsis

A bloclung microcytotoxicity assay was used to detect soluble astrocytoma-associated antigen. The richest source of soluble antigen was found in spent culture media from an established glioblastoma (GF) tissue culture line. Also assayed were fractions of sonicated membrane antigen from another (GM) glioblastoma and pellets of GF and GM cultured glioblastoma tissue. Bloclung by media conditioned by cultured normal human brain, breast cancer, neuroblastoma, meningioma, or 2-year-old astrocytoma cell lines was 41-82% lower. A monomer was isolated that blocked cytotoxicity and migrated in molecular exclusion chromatography with alpha-macroglobulins rather than the beta-2-microglobulins usually associated with histocompatibility antigens.

๐Ÿ“œ SIMILAR VOLUMES

Rhabdomyosarcoma cell line can be used f
Rhabdomyosarcoma cell line can be used for the isolation of soluble acetylcholine receptor and for assaying blocking and modulating autoantibodies
โœ James T. Wu; Mark Astill; Christopher Lloyd; V. C. Salmon ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 742 KB

## Abstract We found that the Rhabdomyosarcoma (RD) cell line expresses human acetylcholine receptor (AChR) based on the following evidences: 1. Soluble AChR can be isolated from RD cells following the isolation procedure for AChR from human muscle; 2. Intact RD cells bind to alphaโ€bungarotoxin (ฮฑB

HCV core antigen and combination (antige
HCV core antigen and combination (antigen/antibody) assays for the detection of early seroconversion
โœ George J. Dawson ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 84 KB

Detection of antibodies against hepatitis C virus (HCV) and/or detection of HCV RNA by nucleic acid testing (NAT) are the commonly utilized methods for detecting exposure to HCV in both blood screening and diagnostic laboratories. While several HCV core antigen tests have been developed over the las

Studies on epstein-barr virus-related an
Studies on epstein-barr virus-related antigens. I. Indirect single radial immunodiffusion as a useful method for detection and assay of soluble antigen
โœ T. Matsuo; T. Osato; S. Nishi; H. Hirai ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 461 KB ๐Ÿ‘ 1 views

## Abstract A useful method for the detection and assay of Epsteinโ€Barr virus (EBV)โ€related soluble antigen has been developed by the application of the indirect single radial immunodiffusion technique which is frequently used for quantitative measurements of immunoglobulins and other soluble prote

Enzyme-Linked immunosorbent assay for th
Enzyme-Linked immunosorbent assay for the detection and identification of coxsackie b antigen in tissue cultures and clinical specimens
โœ Robert H. Yolken; Virginia Torsch ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 416 KB

## Abstract An enzymeโ€linked immunosorbent assay (ELISA) has been developed for the identification of Coxsackie B antigens. This assay was capable of identifying and distinguishing all six Coxsackie B serotypes at concentrations one hundredfold to ten thousandfold less than could be detected by com

An enzyme-linked immunosorbent assay for
An enzyme-linked immunosorbent assay for the detection of antitetanus toxoid antibody using aluminum-absorbed coating antigen
โœ G. M. Thiele; J. Rogers; M. Collins; D. Smith; T. L. McDonald; N. Yasuda ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 375 KB

## Abstract An enzymeโ€linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminumโ€absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunizati