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Microcentrifuge desalting: A rapid, quantitative method for desalting small amounts of protein

✍ Scribed by E. Helmerhorst; G.B. Stokes

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
457 KB
Volume
104
Category
Article
ISSN
0003-2697

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A simple method for protein recovery from gel slices following polyacrylamide gel electrophoresis is proposed which requires neither additional devices nor much skill. The method is based on reversed electrophoresis using a discontinuous conductivity gradient which is in turn maintained by a gradien