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Microcalorimetric Studies on the Interaction Mechanism between Proteins and Hydrophobic Solid Surfaces in Hydrophobic Interaction Chromatography: Effects of Salts, Hydrophobicity of the Sorbent, and Structure of the Protein

โœ Scribed by Lin, Fu-Yung; Chen, Wen-Yih


Book ID
127211851
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
110 KB
Volume
73
Category
Article
ISSN
0003-2700

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Isothermal Titration Microcalorimetric S
โœ Hsiang-Ming Huang; Fu-Yung Lin; Wen-Yih Chen; Ruoh-Chyu Ruaan ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 93 KB

This study attempted to comprehend how temperature affects hydrophobic interaction between proteins and hydrophobic adsorbents. By equilibrium batch analysis, we measured the adsorption isotherm to evaluate the protein-adsorbent affinity, while isothermal titration calorimetry was used to measure th