Microbore liquid chromatography and refractive index gradient detection of low-nanogram and low-ppm quantities of carbohydrates

✍ Scribed by Darrell O. Hancock; Robert E. Synovec

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 633 KB
• Volume: 464
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94225-0

✦ Synopsis

A refractive index gradient detector is presented as a universal detector in the microbore high-performance liquid chromatography analysis of carbohydrates. Simultaneously, low-ng and low-ppm injected quantities of carbohydrates were detected at the 3 x root-mean-square baseline noise level. A typical microbore high-performance liquid chromatography chromatogram separating fructose from sucrose followed by refractive index gradient (RIG) detection is reported. Use of a position sensitive detector (PSD) in the RIG detector design is reported and experimental considerations discussed. Optimization of the PSD-based RIG detector is addressed. Potential for the device in industrial and clinical applications is considered.