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Microbiological implications of electric field effects: Part VIII. Fusion of Bacillus thuringiensis protoplasts by high electric field pulses

✍ Scribed by N. Shivarova; R. Grigorova; W. Förstes; H.-E. Jacob; H. Berg

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
319 KB
Volume
11
Category
Article
ISSN
1567-5394

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## Abstract Prototrophic colonies could be selected on minimal medium after mixing of protoplasts from diauxotrophic mutants of the yeasts __Saccharomycopsis lipolytica__ and/or __Lodderomyces elongisporus__ and treatment with polyethylene glycol (PEG) in the presence of calcium chloride. This is t