Microbial transformation of steroids XVIII. Dehydrogenation of cortisone in position 1—2
✍ Scribed by A. čapek; O. Hanč; B. Kakač; M. Tadra
- Book ID
- 112941316
- Publisher
- Springer Netherlands
- Year
- 1962
- Tongue
- English
- Weight
- 406 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0015-5632
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Fungal cells from Curnularia Zunala were entrapped in a crosslinked polyacrylamide gel. The gel-cells obtained aa granules were applied in the microbial transformation of Reichstein compound S leading to cortisol through an 11-,6hydroxylation step. Some kinetic studies of this conversion using gel-c
Art/irohcrcter osyciuns 3 17 capable of complete degradation of 0-silosterol harbours two plasmids -pJLI and pRLI. Standard plasmid isolation methods can easily detect pJL1 but not pRLI. The latter plasmid was isolated by a mild procedure using a,a'-dipyridyl as lytic agent and separated from pJLI b