Preparation and application of polymer-entrapped enzymes and microorganisms in microbial transformation processes with special reference to steroid 11-β-hydroxylation and Δ1-dehydrogenation
✍ Scribed by Klaus Mosbach; Per-Olof Larsson
- Publisher
- John Wiley and Sons
- Year
- 1970
- Tongue
- English
- Weight
- 439 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Fungal cells from Curnularia Zunala were entrapped in a crosslinked polyacrylamide gel. The gel-cells obtained aa granules were applied in the microbial transformation of Reichstein compound S leading to cortisol through an 11-,6hydroxylation step. Some kinetic studies of this conversion using gel-cells were carried out. In addition, it waa shown that gel-cell granules which had lost part of their 11-phydroxylsse activity on storage could be reactivated yielding preparations with increased activity.
From Corynebacterium simpbz a steroid dehydrogenase catalyzing the A1dehydrogenation of cortisol leading to predniiolone w a ~ isolated and partially purified. The enzyme preparation waa entrapped in a crosslinked polyacrylaniide gel and the gel-enzyme granules obtained used in steroid dehydrogenation processes.