Microbial Gene Essentiality: Protocols and Bioinformatics

✍ Scribed by Karen Joy Shaw (auth.), Andrei L. Osterman, Svetlana Y. Gerdes (eds.)

• Publisher: Humana Press
• Year: 2008
• Tongue: English
• Leaves: 481
• Series: Methods in Molecular Biology 416
• Edition: 1
• Category: Library

✦ Synopsis

Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.

✦ Table of Contents

Front Matter....Pages i-xiv
Front Matter....Pages 1-1
Front Matter....Pages 3-8
Front Matter....Pages 9-11
Front Matter....Pages 13-26
Back Matter....Pages 27-44
....Pages 45-59

✦ Subjects

Cell Biology; Human Genetics