Gene Essentiality: Methods and Protocols

✍ Scribed by Long Jason Lu (eds.)

Publisher: Humana Press
Year: 2015
Tongue: English
Leaves: 247
Series: Methods in Molecular Biology 1279
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.

✦ Table of Contents

Front Matter....Pages i-xi
Back Matter....Pages 1-14
....Pages 15-23

✦ Subjects

Human Genetics; Microbial Genetics and Genomics

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