Microassay of cysteine sulfinic acid by an enzymatic cycling method

✍ Scribed by Akemichi Basa; Satoru Yamagami; Hiroyuki Mizuo; Heitaroh Iwata

• Book ID: 102626020
• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 395 KB
• Volume: 101
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90188-8

✦ Synopsis

A method for microassay of cysteine sulfinic acid (CSA) was developed. The principle of this method is the enzymatic conversion of CSA to lactate and the subsequent enzymatic cycling of NAD, which is a final by-product of the enzymatic conversion system. Aspartate, pyruvate, and NAD, which cause interference, were eliminated by two procedures. The absorbance of NADH was linearly proportional to the concentration of CSA over the concentration range of 5-50 pmol and CSA could be measured in as little as 50 pg of rat brain tissue. The regional and subcellular distributions of CSA in rat brain were studied.