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An enzymatic cycling method for the determination of serum total bile acids with recombinant 3α-hydroxysteroid dehydrogenase

✍ Scribed by Guo-Hua Zhang; Ai-Ri Cong; Guo-Bing Xu; Chuan-Bao Li; Rui-Feng Yang; Tie-An Xia


Book ID
116289550
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
460 KB
Volume
326
Category
Article
ISSN
0006-291X

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A method is described for the separation of unconjugated bile acids and their glycine and taurine conjugates in a single step with adequate sensitivity for analysis of serum samples. Separation was carried out over a period of 80 min using a linear gradient with increasing concentrations of methanol