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Enzymatic determination of serum 12α-hydroxy bile acid concentration with 12α-hydroxysteroid dehydrogenase

✍ Scribed by Tamasawa, Naoki ;Yoneda, Masashi ;Makino, Isao ;Takebe, Kazuo ;Ueda, Shigeru ;Misaki, Hideo


Publisher
Springer-Verlag
Year
1988
Tongue
English
Weight
439 KB
Volume
23
Category
Article
ISSN
0435-1339

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A method is described for the separation of unconjugated bile acids and their glycine and taurine conjugates in a single step with adequate sensitivity for analysis of serum samples. Separation was carried out over a period of 80 min using a linear gradient with increasing concentrations of methanol