Enzymatic determination of serum 3α-sulfated bile acids concentration with bile acid 3α-sulfate sulfohydrolase
✍ Scribed by Takafumi Kato; Masashi Yoneda; Kimihide Nakamura; Isao Makino
- Publisher
- Springer US
- Year
- 1996
- Tongue
- English
- Weight
- 768 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0163-2116
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A method for the separatory determination of bile acid 3-sulfates by liquid chromatography (LC)/electrospray ionization (ESI) mass spectrometry (MS) was developed. The sulfates were characterized by an abundant pseudomolecular ion [ M Ô H ]with a doubly charged ion [ M Ô 2H ] 2-, and the ratio of th
tive pathway bypasses cholesterol 7a-hydroxylase activity. Serum concentrations of 7a-hydroxy-4-cholesten-3-Studies on rat and human hepatocytes in cell culture indicate one (a-HC) have recently been shown to reflect the activthat bile acid synthesis from 27-hydroxycholesterol might acity of cholest
A method is described for the separation of unconjugated bile acids and their glycine and taurine conjugates in a single step with adequate sensitivity for analysis of serum samples. Separation was carried out over a period of 80 min using a linear gradient with increasing concentrations of methanol