Microarrays: Volume I: Synthesis Methods (Methods in Molecular Biology, v381)

✍ Scribed by Jang B. Rampal

• Publisher: Humana Press
• Year: 2007
• Tongue: English
• Leaves: 469
• Series: Methods in Molecular Biology volume 381
• Edition: 2nd
• Category: Library

✦ Synopsis

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes. This is done while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.

✦ Table of Contents

CoverPage_978-1-58829-589-7......Page 1
FrontMatter......Page 2
MMB Series......Page 3
TitlePage......Page 4
CopyRight......Page 5
Dedication......Page 6
Preface......Page 8
Contents......Page 12
Contents of the Sister Volume......Page 14
Contributors......Page 18
1 Introduction......Page 22
2 Current Microarray Surface Chemistries......Page 58
3 Nonfouling Surfaces......Page 80
4 Optimization of Oligonucleotide DNA Microarrays......Page 114
5 Detection of DNA Copy-Number Alterations in ComplexGenomes......Page 126
6 Evaluating the Quality of Data From DNA MicroarrayMeasurements......Page 142
7 Construction of Oligonucleotide Microarrays (Biochip)Using Heterobifunctional Reagents......Page 154
8 Choice of Polymer Matrix, Its Functionalizationand Estimation of Functional Group Density......Page 186
9 Methods in High-Resolution, Array-Based ComparativeGenomic Hybridization......Page 210
10 Design and Fabrication of Spotted Long OligonucleotideMicroarrays for Gene Expression Analysis......Page 234
11 Construction of In Situ Oligonucleotide Arrayson Plastic......Page 248
12 Detecting Ligated Fragmentson Oligonucleotide Microarrays......Page 268
13 Detection of Single-Nucleotide Polymorphismsin Cancer-Related Genes......Page 288
14 Hybridization Analysis Using OligonucleotideProbe Arrays......Page 300
15 In Situ Synthesis of Peptide MicroarraysUsing Ink-Jet Microdispensing......Page 320
16 Intein-Mediated Peptide Arrays for Epitope Mappingand Kinase/Phosphatase Assays......Page 334
17 Printing Low Density Protein Arrays in Microplates......Page 360
18 Forward-Phase and Reverse-Phase Protein Microarray......Page 384
19 Cell Microarray for Functional Exploration of Genomes......Page 396
20 Quantification of Mixed-Phase Hybridization on PolymerMicroparticles......Page 406
21 Measurement of the Sugar-Binding Specificity of Lectins......Page 422
22 Nanotechnology......Page 432
BackMatter_INDEX......Page 458