Microarrays: Volume 2, Applications and Data Analysis (Methods in Molecular Biology, v382)

✍ Scribed by Jang B. Rampal

• Publisher: Humana Press
• Year: 2007
• Tongue: English
• Leaves: 471
• Series: Methods in Molecular Biology volume 382
• Edition: 2nd
• Category: Library

✦ Synopsis

This second part in the two-volume work Microarrays details applications and data analysis. It includes insight into non-mammalian vertebrate systems, processes and protocols for high quality glass-based microarrays. Coverage includes applications in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray platforms in clinical practice, and screening of cDNA libraries on glass slide microarrays. Authors in this volume also discuss protocols for predicting DNA duplex stability on oligonucleotide arrays and integrated analysis of microarray results.

✦ Table of Contents

Cover_978-1-58829-944-4......Page 1
FrontMatter......Page 2
MMB Series......Page 3
TitlePage......Page 4
CopyRight......Page 5
Dedication......Page 6
Preface......Page 8
Contents......Page 12
Contents of the Sister Volume......Page 16
Contributors......Page 18
1 The Use of Microarray Technologyin Nonmammalian Vertebrate Systems......Page 22
2 Quality Considerations and Selection of SurfaceChemistry......Page 38
3 Optimization Workflow for the Processingof High Quality Glass-Based Microarrays......Page 54
4 Processing Protocols for High Quality Glass-BasedMicroarrays......Page 74
5 Specific Detection of Bacterial PathogensUsing Oligonucleotide Microarrays......Page 88
6 Uses of Microarray Platforms in Cancer......Page 98
7 Microarray Technology for Use in Molecular Epidemiology......Page 118
8 Utilization of Microarray Platforms in Clinical Practice......Page 136
9 A Microarray-Based Method to Profile GlobalmicroRNA Expression in Human and Mouse......Page 158
10 Genotyping of Single Nucleotide Polymorphismsby Arrayed Primer Extension......Page 170
11 Protein Chip for Detection of DNA Mutations......Page 184
12 Screening of cDNA Libraries on Glass Slide Microarrays......Page 198
13 ArrayPlex SA......Page 225
14 Tumor–Stroma Interactions of Metastatic ProstateCancer Cell Lines......Page 244
15 Identification of Small Molecule Targetson Functional Protein Microarrays......Page 260
16 Quantification of Small Molecules UsingMicroarray Technology......Page 270
17 Antibody-Microarrays on Hybrid Polymeric ThinFilm-Coated Slides......Page 280
18 Overprint Immunoassay Using Protein A Microarrays......Page 294
19 μParaflo™ Biochip for Nucleic Acid and Protein Analysis......Page 308
20 Application of ProteinChip Array Profilingin Serum Biomarker Discovery......Page 334
21 Volumetric Mass Spectrometry Protein Arrays......Page 354
22 Microarray Data Classifiedby Artificial Neural Networks......Page 366
23 Methods for Microarray Data Analysis......Page 394
24 Predicting DNA Duplex Stability on Oligonucleotide Arrays......Page 414
25 Bioinformatics......Page 426
26 In Silico Gene Selection for Custom OligonucleotideMicroarray Design......Page 438
27 Integrated Analysis of Microarray Results......Page 450
BackMatter_INDEX......Page 460